An educational reference on peptides used in dogs: BPC-157, TB-500, the Wooferine stack, GHK-Cu, and more. Includes weight based dose ranges, reconstitution guidance, protocol templates, and safety notes. Not veterinary advice.
Educational estimate only. Not veterinary advice. Always confirm with a vet before injecting anything into your dog.
Range: 1–2.5 mcg/lb, once daily.
Much of the original BPC-157 and TB-500 research was done in rodents, and the healing mechanisms (angiogenesis, cell migration, growth factor upregulation, collagen remodeling) are conserved across mammals. Veterinarians in regenerative medicine have used these peptides off label for years, particularly for:
None of these peptides are FDA approved for veterinary use. Everything below is off label and should be discussed with your vet.
CCL sprains and post op TPLO, iliopsoas strains, biceps tendinopathy, carpal hyperextension. Typical stack: BPC-157 daily + TB-500 twice weekly for 4–6 weeks, with strict rest for the first 14 days.
Chronic vomiting, diarrhea, or NSAID-induced GI damage. Oral BPC-157 is commonly used here since the peptide is stable in gastric acid. 4 week trial, reassess.
Hot spots, lick granulomas, slow healing surgical incisions, senior coat thinning. GHK-Cu is the workhorse — SubQ or topical on intact skin, never on open wounds without vet guidance.
Arthritic seniors, immune suppressed dogs, dogs recovering from tick borne illness. Thymosin Alpha-1 in short cycles + BPC-157 for joint comfort is a common combo reported by integrative vets.
| Peptide | Common use | Weight-based dose | Frequency | Route |
|---|---|---|---|---|
| BPC-157 | Soft tissue injuries, IBD, leaky gut, post surgical recovery, NSAID damage. | 1–2.5 mcg / lb / day | Once daily, 4–6 week cycle | Subcutaneous (SubQ). Oral form used for gut issues. |
| TB-500 (Thymosin Beta-4) | Chronic or diffuse injuries, muscle recovery, tendon/ligament repair. | ~0.05 mg / lb / week (loading), then half for maintenance | Split into 2 weekly doses during 4–6 week loading, then weekly | Subcutaneous or intramuscular. Systemic, injection site does not matter. |
| Wooferine Stack (BPC-157 + TB-500) | Combined targeted + systemic repair. Common for post op or chronic mobility issues. | BPC-157 at daily dose + TB-500 at weekly dose (see rows above) | BPC-157 daily, TB-500 2x/week, 4–6 weeks | Subcutaneous. Reconstitute separately, can be drawn together. |
| GHK-Cu (Copper Peptide) | Skin, coat, wound healing, hot spots, older-dog collagen support. | ~0.05 mg / lb, 2–3x per week | Cycles of 4–8 weeks | Subcutaneous or topical for skin lesions. |
| LL-37 | Antimicrobial support in chronic infections when guided by a vet. | Highly protocol dependent — do not self-dose | Short cycles only, under veterinary supervision | Subcutaneous. |
| Thymosin Alpha-1 | Immune modulation in older dogs, chronic viral or immune suppressed states. | ~0.015 mg / lb, 2–3x per week | 4–8 week cycles | Subcutaneous. |
The classic BPC-157 + TB-500 combination. Best for post op recovery, chronic soft tissue issues, or older dogs with cumulative wear.
| Dog weight | Approx. daily BPC-157 |
|---|---|
| 10 lb | ~15 mcg / day |
| 25 lb | ~38 mcg / day |
| 50 lb | ~75 mcg / day |
| 75 lb | ~113 mcg / day |
| 100 lb | ~150 mcg / day |
Ranges shown are midpoints. Small dogs often start on the low end (1 mcg/lb); working or injured dogs may run closer to 2.5 mcg/lb under supervision.
Because dogs need far smaller doses than humans, most owners over-dilute on purpose. A larger BAC water volume makes drawing 2–10 unit doses on an insulin syringe practical instead of trying to measure a microscopic line.
A 4 week Wooferine cycle for a 40 lb dog typically uses about one 5 mg BPC-157 vial and one 5 mg TB-500 vial. Vendor pricing varies, but expect roughly $60–$120 total per cycle for research grade product, plus bacteriostatic water and insulin syringes.
Owner and integrative vet reports have been broadly positive for soft tissue and post op use, but there is no long term canine safety trial. Treat it as off label experimental care and monitor closely.
Owners commonly report improved comfort and mobility within 10–14 days on the Wooferine stack. Structural healing takes longer — commit to a full 4–6 week cycle before judging.
Injection (SubQ) is preferred for orthopedic issues because it reaches systemic circulation. Oral is preferred for gut issues since the peptide acts locally on the GI tract.
The molecules are identical — a peptide vial doesn't know the species. What matters is the dose per kg, the injection technique, and product quality. Never use a product you wouldn't put in yourself.
Published canine specific work is thin. Most evidence is rodent preclinical or case series from integrative veterinary practices. This is a rapidly evolving area — check back periodically.
Long term safety data in dogs is thin. Most published research is rodent preclinical work; canine evidence is largely case series and clinical experience from integrative vets. Assume anything on this page could be revised as better data appears.
Educational content only. Not veterinary advice. Consult a licensed veterinarian before giving any peptide to your pet.
BPC-157 and TB-500 amounts for 10–100 lb dogs, unit conversions, and a log to bring to your vet.
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