Free Reference

Peptide reconstitution & mixing chart

A printable cheat sheet for mixing lyophilized peptide with bacteriostatic water. Find the right BAC volume for your vial size and convert syringe units to micrograms in one glance.

How to read this chart

  1. 1Find your vial size in the first column (2mg, 5mg, or 10mg).
  2. 2Pick your BAC water volume (1mL, 2mL, or 3mL). More water lowers the concentration, which means more units per dose.
  3. 3Read the concentration for that combination, then use the units-to-mcg table to see how many units to draw for your target dose.

Concentration cheat sheet

Concentration in micrograms per milliliter (mcg/mL) after reconstitution. Values assume the full vial amount is dissolved in the listed BAC water volume.

Vial size1 mL BAC2 mL BAC3 mL BAC
2 mg2,000 mcg/mL(2.00 mg/mL)1,000 mcg/mL(1.00 mg/mL)666.667 mcg/mL(0.67 mg/mL)
5 mg5,000 mcg/mL(5.00 mg/mL)2,500 mcg/mL(2.50 mg/mL)1,666.667 mcg/mL(1.67 mg/mL)
10 mg10,000 mcg/mL(10.00 mg/mL)5,000 mcg/mL(5.00 mg/mL)3,333.333 mcg/mL(3.33 mg/mL)

Units to micrograms lookup

For each vial + BAC combination, the table below shows how many units to draw on a standard U-100 insulin syringe for common research doses.

2 mg vial

BAC water100 mcg250 mcg500 mcg1.0 mg
1 mL5.0 units12.5 units25.0 units50.0 units
2 mL10.0 units25.0 units50.0 units100.0 units
3 mL15.0 units37.5 units75.0 units

5 mg vial

BAC water100 mcg250 mcg500 mcg1.0 mg
1 mL2.0 units5.0 units10.0 units20.0 units
2 mL4.0 units10.0 units20.0 units40.0 units
3 mL6.0 units15.0 units30.0 units60.0 units

10 mg vial

BAC water100 mcg250 mcg500 mcg1.0 mg
1 mL1.0 units2.5 units5.0 units10.0 units
2 mL2.0 units5.0 units10.0 units20.0 units
3 mL3.0 units7.5 units15.0 units30.0 units

Quick reconstitution steps

  1. 1Swab both stoppers with alcohol pads and let them air dry.
  2. 2Draw the BAC water into a reconstitution syringe, then inject it slowly down the inside wall of the peptide vial.
  3. 3Swirl gently until the powder is fully dissolved. Never shake, foaming can damage the peptide.
  4. 4Draw your dose on a U-100 insulin syringe using the units shown in the chart above.
  5. 5Store refrigerated at 2–8 °C (36–46 °F). Most reconstituted peptides remain stable for 3–4 weeks.

Why this matters

Getting the concentration right is the most common mistake new researchers make. Too much water and a single unit becomes a tiny dose; too little water and drawing accurately becomes difficult. This chart removes the guesswork so you can focus on protocol consistency.

Use the interactive calculator
Disclaimer: This chart is for educational and research reference only. Peptides listed are not FDA approved for human consumption. Always consult a licensed healthcare professional before use, verify third party COAs, and follow sterile handling practices.
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