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Mitochondrial

SS-31 dosing guide

Elamipretide — the mitochondrial bodyguard that binds cardiolipin.. Everything below is the arithmetic of this vial — what to mix, what to draw, how often, and for how long.

Also sold as: ss31, ss 31, elamipretide, mtp-131

The protocol at a glance

Vial size
10 mg
Bacteriostatic water
2 mL
Concentration
5.00 mg/mL
Dose
1 mg
Draw on a 1 mL syringe
20 units
Timing
AM
Frequency
5 days on, 2 days off
Cycle length
8 weeks on, 8 weeks off
Doses per vial
about 10
Draw to here
00.0100.1200.2300.3400.4500.5600.6700.7800.8900.91001.0DRAW TO 20u100-UNIT · 1 mL SYRINGESS-31
Change the vial or water volume in the calculator →

Mixing it, step by step

Step by step

Reconstitution steps

Every step below uses the numbers you entered above.

  1. 1
    Wipe both stoppers

    Swab the rubber tops of the 10 mg SS-31 vial and the bacteriostatic water vial with fresh alcohol pads. Let them air dry for a few seconds.

  2. 2
    Draw 2 mL of BAC water

    Pull 2 mL of bacteriostatic water into a reconstitution syringe (usually a 3 mL syringe with a 21g needle).

  3. 3
    Add water slowly, down the side

    Inject the water against the inside wall of the vial, don't blast it directly onto the powder. This protects the peptide from shear damage.

  4. 4
    Swirl, don't shake

    Gently swirl until the powder is fully dissolved and the liquid is clear. Never shake, foaming denatures the peptide.

  5. 5
    Draw 20 units on a 1 mL syringe

    On your 100-unit (1 mL) insulin syringe, pull the plunger back until the rubber tip lines up with 20, that's your 1.00 mg dose. Tap out any air bubbles, then inject as directed.

  6. 6
    Store refrigerated

    Cap the vial and store at 2–8 °C (36–46 °F). Most reconstituted peptides remain stable for 3–4 weeks refrigerated.

What to expect, week by week

Week 1

Energy floor lifts. Fewer afternoon crashes. Workouts feel less taxing.

Weeks 2–4

Endurance and recovery both improve. Some users report better glucose control.

Weeks 4–8

Peak effect. Consider a break so mitochondrial adaptation is preserved, not blunted.

Side effects to watch

  • Mild injection site reaction
  • Rare GI upset

Why people run it

  • Protects mitochondrial function
  • Reduces cardiac apoptosis
  • Improves muscle strength in mito disease

What to stack it with

Ready to run SS-31?

Read the full compound page, or check current pricing.

Educational reference only. Doses shown reflect commonly reported research protocols and are not medical advice.

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