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Wellness

B12 dosing guide

The most bioavailable B12 form, delivered where the gut can't limit it.. Everything below is the arithmetic of this vial — what to mix, what to draw, how often, and for how long.

Also sold as: b12, b-12, vitamin b12, methyl b12, methylcobalamin, cobalamin

The protocol at a glance

Vial size
10 mg
Bacteriostatic water
10 mL
Concentration
1.00 mg/mL
Dose
1 mg
Draw on a 1 mL syringe
10 units
Timing
AM
Frequency
2–3x per week
Cycle length
Ongoing
Doses per vial
about 10

Pre-mixed as 1mg/mL. IM or SubQ. Retest B12 + homocysteine at 8 weeks.

Draw to here
00.0100.1200.2300.3400.4500.5600.6700.7800.8900.91001.0DRAW TO 10u100-UNIT · 1 mL SYRINGEB12
Change the vial or water volume in the calculator →

Mixing it, step by step

Step by step

Reconstitution steps

Every step below uses the numbers you entered above.

  1. 1
    Wipe both stoppers

    Swab the rubber tops of the 10 mg B12 vial and the bacteriostatic water vial with fresh alcohol pads. Let them air dry for a few seconds.

  2. 2
    Draw 10 mL of BAC water

    Pull 10 mL of bacteriostatic water into a reconstitution syringe (usually a 3 mL syringe with a 21g needle).

  3. 3
    Add water slowly, down the side

    Inject the water against the inside wall of the vial, don't blast it directly onto the powder. This protects the peptide from shear damage.

  4. 4
    Swirl, don't shake

    Gently swirl until the powder is fully dissolved and the liquid is clear. Never shake, foaming denatures the peptide.

  5. 5
    Draw 10 units on a 1 mL syringe

    On your 100-unit (1 mL) insulin syringe, pull the plunger back until the rubber tip lines up with 10, that's your 1.00 mg dose. Tap out any air bubbles, then inject as directed.

  6. 6
    Store refrigerated

    Cap the vial and store at 2–8 °C (36–46 °F). Most reconstituted peptides remain stable for 3–4 weeks refrigerated.

What to expect, week by week

Week 1

Baseline shift. Energy, sleep, or mood may improve within days depending on the compound and prior deficiency.

Weeks 2–4

Full effect stabilizes. Recheck bloodwork (B12, ferritin, homocysteine) where relevant to confirm response.

Ongoing

Most wellness injectables are run indefinitely at a maintenance cadence, cycled only if labs normalize.

Side effects to watch

  • Injection site soreness
  • Rare acne flare
  • Bright yellow urine (harmless)

Why people run it

  • Corrects deficiency fatigue
  • Supports myelin and nerve repair
  • Lowers homocysteine with folate
  • Supports red blood cell formation

Ready to run B12?

Read the full compound page, or check current pricing.

Educational reference only. Doses shown reflect commonly reported research protocols and are not medical advice.

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