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Healing

TB-500 dosing guide

The cell migration half of the healing equation.. Everything below is the arithmetic of this vial — what to mix, what to draw, how often, and for how long.

Also sold as: tb500, tb 500, thymosin beta 4, tb4

The protocol at a glance

Vial size
10 mg
Bacteriostatic water
2 mL
Concentration
5.00 mg/mL
Dose
2.5 mg
Draw on a 1 mL syringe
50 units
Timing
AM
Frequency
2x per week
Cycle length
Load 4–6 weeks, then 2.5mg weekly maintenance
Doses per vial
about 4

Loading: 2.5mg twice weekly for 4–6 weeks; maintenance: 2.5mg once weekly.

Draw to here
00.0100.1200.2300.3400.4500.5600.6700.7800.8900.91001.0DRAW TO 50u100-UNIT · 1 mL SYRINGETB-500
Change the vial or water volume in the calculator →

Mixing it, step by step

Step by step

Reconstitution steps

Every step below uses the numbers you entered above.

  1. 1
    Wipe both stoppers

    Swab the rubber tops of the 10 mg TB-500 vial and the bacteriostatic water vial with fresh alcohol pads. Let them air dry for a few seconds.

  2. 2
    Draw 2 mL of BAC water

    Pull 2 mL of bacteriostatic water into a reconstitution syringe (usually a 3 mL syringe with a 21g needle).

  3. 3
    Add water slowly, down the side

    Inject the water against the inside wall of the vial, don't blast it directly onto the powder. This protects the peptide from shear damage.

  4. 4
    Swirl, don't shake

    Gently swirl until the powder is fully dissolved and the liquid is clear. Never shake, foaming denatures the peptide.

  5. 5
    Draw 50 units on a 1 mL syringe

    On your 100-unit (1 mL) insulin syringe, pull the plunger back until the rubber tip lines up with 50, that's your 2.50 mg dose. Tap out any air bubbles, then inject as directed.

  6. 6
    Store refrigerated

    Cap the vial and store at 2–8 °C (36–46 °F). Most reconstituted peptides remain stable for 3–4 weeks refrigerated.

What to expect, week by week

Week 1

Subtle. You may notice slightly less pain or better range of motion. Don't push injuries yet.

Weeks 2–4

Clear improvement in pain and tissue tolerance. Begin controlled loading of the injured area.

Weeks 4–8

Peak repair phase. Function returns. Keep dose consistent — skipping days slows the curve.

Post-cycle

Gains generally stick if you also fixed the underlying mechanics (form, load management, mobility).

Side effects to watch

  • Mild lethargy early in cycle
  • Rare head rush feeling

Why people run it

  • Systemic healing
  • Cell migration to injury
  • Hair regrowth signal
  • Improves flexibility

What to stack it with

Ready to run TB-500?

Read the full compound page, or check current pricing.

Educational reference only. Doses shown reflect commonly reported research protocols and are not medical advice.

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