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Mitochondrial

NAD+ dosing guide

Cellular currency — the cofactor every sirtuin and PARP enzyme depends on.. Everything below is the arithmetic of this vial — what to mix, what to draw, how often, and for how long.

Also sold as: nad+, nad plus, nicotinamide, nicotinamide adenine dinucleotide

The protocol at a glance

Vial size
1000 mg
Bacteriostatic water
4 mL
Concentration
250.00 mg/mL
Dose
75 mg
Draw on a 1 mL syringe
30 units
Timing
AM
Frequency
2–3 days per week
Cycle length
As needed
Doses per vial
about 13
Draw to here
00.0100.1200.2300.3400.4500.5600.6700.7800.8900.91001.0DRAW TO 30u100-UNIT · 1 mL SYRINGENAD+
Change the vial or water volume in the calculator →

Mixing it, step by step

Step by step

Reconstitution steps

Every step below uses the numbers you entered above.

  1. 1
    Wipe both stoppers

    Swab the rubber tops of the 1000 mg NAD+ vial and the bacteriostatic water vial with fresh alcohol pads. Let them air dry for a few seconds.

  2. 2
    Draw 4 mL of BAC water

    Pull 4 mL of bacteriostatic water into a reconstitution syringe (usually a 3 mL syringe with a 21g needle).

  3. 3
    Add water slowly, down the side

    Inject the water against the inside wall of the vial, don't blast it directly onto the powder. This protects the peptide from shear damage.

  4. 4
    Swirl, don't shake

    Gently swirl until the powder is fully dissolved and the liquid is clear. Never shake, foaming denatures the peptide.

  5. 5
    Draw 30 units on a 1 mL syringe

    On your 100-unit (1 mL) insulin syringe, pull the plunger back until the rubber tip lines up with 30, that's your 75.00 mg dose. Tap out any air bubbles, then inject as directed.

  6. 6
    Store refrigerated

    Cap the vial and store at 2–8 °C (36–46 °F). Most reconstituted peptides remain stable for 3–4 weeks refrigerated.

What to expect, week by week

Week 1

Energy floor lifts. Fewer afternoon crashes. Workouts feel less taxing.

Weeks 2–4

Endurance and recovery both improve. Some users report better glucose control.

Weeks 4–8

Peak effect. Consider a break so mitochondrial adaptation is preserved, not blunted.

Side effects to watch

  • Chest/face flushing if pushed fast
  • Nausea
  • Stomach cramping at IV speed

Why people run it

  • Cellular energy production
  • DNA repair via PARPs
  • Sirtuin driven longevity signaling
  • Cognitive clarity

What to stack it with

Ready to run NAD+?

Read the full compound page, or check current pricing.

Educational reference only. Doses shown reflect commonly reported research protocols and are not medical advice.

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